We consider defects in 5d field theories corresponding to higher-rank generalizations of the $E_{N_f+1}$ theories; holographically dual to the Brandhuber-Oz background in type I String Theory. We concentrate on codimension 2 and 1 defects, corresponding, respectively, to 3d and 4d defect Quantum Field Theories. We study holographically such defect theories by considering supersymmetric probe D4- and D6-branes in the $AdS_6$, whose fluctuations allow us to study the spectrum of mesonic operators of the defect theories. In the case of D4-branes, we also consider wrappings in the internal space which can be regarded as generalizations of the configurations capturing the antisymmetric Wilson loop.
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