In this paper, a notion of affine walled Brauer-Clifford superalgebras $BC_{r, t}^{rm aff} $ is introduced over an arbitrary integral domain $R$ containing $2^{-1}$. These superalgebras can be considered as affinization of walled Brauer superalgebras in cite{JK}. By constructing infinite many homomorphisms from $BC_{r, t}^{rm aff}$ to a class of level two walled Brauer-Clifford superagebras over $mathbb C$, we prove that $BC_{r, t}^{rm aff} $ is free over $R$ with infinite rank. We explain that any finite dimensional irreducible $BC_{r, t}^{rm aff} $-module over an algebraically closed field $F$ of characteristic not $2$ factors through a cyclotomic quotient of $BC_{r, t}^{rm aff} $, called a cyclotomic (or level $k$) walled Brauer-Clifford superalgebra $ BC_{k, r, t}$. Using a previous method on cyclotomic walled Brauer algebras in cite{RSu1}, we prove that $BC_{k, r, t}$ is free over $R$ with super rank $(k^{r+t}2^{r+t-1} (r+t)!, k^{r+t}2^{r+t-1} (r+t)!)$ if and only if it is admissible in the sense of Definition~6.4. Finally, we prove that the degenerate affine (resp., cyclotomic) walled Brauer-Clifford superalgebras defined by Comes-Kujawa in cite{CK} are isomorphic to our affine (resp., cyclotomic) walled Brauer-Clifford superalgebras.
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