Let $G$ be a linear algebraic group over an algebraically closed field of characteristic $pgeq 0$. We show that if $H_1$ and $H_2$ are connected subgroups of $G$ such that $H_1$ and $H_2$ have a common maximal unipotent subgroup and $H_1/R_u(H_1)$ and $H_2/R_u(H_2)$ are semisimple, then $H_1$ and $H_2$ are $G$-conjugate. Moreover, we show that if $H$ is a semisimple linear algebraic group with maximal unipotent subgroup $U$ then for any algebraic group homomorphism $sigmacolon Urightarrow G$, there are only finitely many $G$-conjugacy classes of algebraic group homomorphisms $rhocolon Hrightarrow G$ such that $rho|_U$ is $G$-conjugate to $sigma$. This answers an analogue for connected algebraic groups of a question of B. Kulshammer. In Kulshammers original question, $H$ is replaced by a finite group and $U$ by a Sylow $p$-subgroup of $H$; the answer is then known to be no in general. We obtain some results in the general case when $H$ is non-connected and has positive dimension. Along the way, we prove existence and conjugacy results for maximal unipotent subgroups of non-connected linear algebraic groups. When $G$ is reductive, we formulate Kulshammers question and related conjugacy problems in terms of the nonabelian 1-cohomology of unipotent radicals of parabolic subgroups of $G$, and we give some applications of this cohomological approach. In particular, we analyse the case when $G$ is a semisimple group of rank 2.