Motivated by the two-parameter free unitary Segal-Bargmann transform in the form of conditional expectation, we derive the integral transform representation of the two-parameter free unitary Segal-Bargmann transform which coincides to the large-$N$ limit of the two-parameter Segal-Bargmann transform on the unitary group $mathbb{U}(N)$ and explore its limiting behavior. We also extend the notion of circular systems in order to define a two-parameter free Segal-Bargmann transform and prove a version of Biane-Gross-Malliavin Theorem of the two-parameter free unitary Segal-Bargmann transform.