In addition to chemical and mechanical interactions between cells electromagnetic field produced by cells has been considered as another form of signaling for cell-cell communication. The aim of this study is evaluation of electromagnetic effects on viability of Adipose-derived stem cells (ADSCs) without co-culturing. In this study, stem cells were isolated from human adipose tissue enzymatically and proliferated in monolayer culture. Then, 5.(10^4) adipose-derived stem cells were cultured in each well of the test plate. In the first row (4 wells), ADSCs as inducer cells were cultured in DMEM1 with 10 ng/ml Fibroblast growth factor (FGF). In adjacent and the last rows, ADSCs were cultured without FGF (as detector cells). After the three and five days the viability of cells were evaluated. Moreover, ADSCs were cultured in the same conditions but the inducer cells were placed once in the UV-filter tube and once in the quartz tube to see whether there is electromagnetic interaction among cells. Inducer cells caused significant cell proliferation in adjacent row cells (p- value<0.01) in the fifth day. However, using the UV-filter tube and quartz tube both reduced the effect of inducer cells on adjacent cells significantly. As a conclusion, we could detect distant cellular interaction (DCI) among adipose derived stem cells (ADSCs), but it was not electromagnetic signaling. Our results show that ADSCs affect each other via volatile signaling as a chemical distant cellular interaction (CDCI).
تحميل البحث