Block sensitivity ($bs(f)$), certificate complexity ($C(f)$) and fractional certificate complexity ($C^*(f)$) are three fundamental combinatorial measures of complexity of a boolean function $f$. It has long been known that $bs(f) leq C^{ast}(f) leq C(f) =O(bs(f)^2)$. We provide an infinite family of examples for which $C(f)$ grows quadratically in $C^{ast}(f)$ (and also $bs(f)$) giving optimal separations between these measures. Previously the biggest separation known was $C(f)=C^{ast}(f)^{log_{4.5}5}$. We also give a family of examples for which $C^{ast}(f)=Omega(bs(f)^{3/2})$. These examples are obtained by composing boolean functions in various ways. Here the composition $f circ g$ of $f$ with $g$ is obtained by substituting for each variable of $f$ a copy of $g$ on disjoint sets of variables. To construct and analyse these examples we systematically investigate the behaviour under function composition of these measures and also the sensitivity measure $s(f)$. The measures $s(f)$, $C(f)$ and $C^{ast}(f)$ behave nicely under composition: they are submultiplicative (where measure $m$ is submultiplicative if $m(f circ g) leq m(f)m(g)$) with equality holding under some fairly general conditions. The measure $bs(f)$ is qualitatively different: it is not submultiplicative. This qualitative difference was not noticed in the previous literature and we correct some errors that appeared in previous papers. We define the composition limit of a measure $m$ at function $f$, $m^{lim}(f)$ to be the limit as $k$ grows of $m(f^{(k)})^{1/k}$, where $f^{(k)}$ is the iterated composition of $f$ with itself $k$-times. For any function $f$ we show that $bs^{lim}(f) = (C^*)^{lim}(f)$ and characterize $s^{lim}(f), (C^*)^{lim}(f)$, and $C^{lim}(f)$ in terms of the largest eigenvalue of a certain set of $2times 2$ matrices associated with $f$.
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