A flux-calibrated high resolution spectrum of the airglow emission is a practical lambda-calibration reference for astronomical spectral observations. It is also useful for constraining the molecular parameters of the OH molecule and the physical conditions in the upper mesosphere. methods: We use the data collected during the first technical commissioning of the GIANO spectrograph at the Telescopio Nazionale Galileo (TNG). The high resolution (R~50,000) spectrum simultaneously covers the 0.95-2.4 micron wavelength range. Relative flux calibration is achieved by the simultaneous observation of spectrophotometric standard star. results: We derive a list of improved positions and intensities of OH infrared lines. The list includes Lambda-split doublets many of which are spectrally resolved. Compared to previous works, the new results correct errors in the wavelengths of the Q-branch transitions. The relative fluxes of OH lines from different vibrational bands show remarkable deviations from theoretical predictions: the Deltav=3,4 lines are a factor of 2 and 4 brighter than expected. We also find evidence of a significant fraction (1-4%) of OH molecules with ``non-thermal population of high-J levels. Finally we list wavelengths and fluxes of 153 lines not attributable to OH. Most of these can be associated to O2, while 37 lines in the H band are not identified. The O2 and unidentified lines in the H band account for ~5% of the total airglow flux in this band.
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