In this paper, we presented metal abundance properties of 144 M81 globular clusters. These globulars consist of the largest globular cluster sample in M81 till now. Our main results are: the distribution of metallicities are bimodal, with metallicity peaks at [Fe/H]sim-1.51 and -0.58, and the metal-poor globular clusters tend to be less spatially concentrated than the metal-rich ones; the metal-rich globular clusters in M81 do not demonstrate a centrally concentrated spatial distribution as the metal-rich ones in M31 do; like our Galaxy and M31, the globular clusters in M81 have a small radial metallicity gradient. These results are consistent with those obtained based on a small sample of M81 globular clusters. In addition, this paper showed that there is evidence that a strong rotation of the M81 globular cluster system around the minor axis exists, and that rotation is present in the metal-rich globular cluster subsample, while the metal-poor globular cluster subsample shows no evidence for rotation. The most significant difference between the rotation of the metal-rich and metal-poor globular clusters occurs at intermediate projected galactocentric radii. The results of this paper confirm the conclusion of Schroder et al. that M81s metal-rich globular clusters at intermediate projected radii were associated with a thick disk of M81.
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