In this paper, the conjugate-linear anti-involutions and the unitary irreducible modules of the intermediate series over the twisted Heisenberg-Virasoro algebra are classified respectively. We prove that any unitary irreducible module of the intermediate series over the twisted Heisenberg-Virasoro algebra is of the form $mathcal{A}_{a,b,c}$ for $ain mathbb{R}, bin 1/2+sqrt{-1}mathbb{R}, cin mathbb{C}.$
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