Multiple populations have been detected in several globular clusters (GC) that do not display a spread in metallicity. Unusual features of their CMD can be interpreted in terms of differences in the Helium content of the stars belonging to the sub-populations. Differences in He abundance have never been directly observed. We attempt to measure these differences in two giant stars of NGC 2808 with very similar parameters but different Na and O abundances, hence that presumably belong to different sub-populations, by directly comparing their He I 10830 {AA} lines. The He 10830 {AA} line forms in the upper chromosphere. Our detailed models derive the chromospheric structure using the Ca II and H$alpha$, and simulate the corresponding He I 10830 line profiles. We show that, at a given value of He abundance, the He I 10830 equivalent width cannot significantly change without a corresponding much larger change in the Ca II lines. We have used the VLT-CRIRES to obtain high-resolution spectra in the 10830 {AA} region, and the VLT-UVES to obtain spectra of the Ca II and H$alpha$ lines of our target stars. The two target stars have very similar Ca II and H$alpha$ lines, but different appearances in the He region. One line, blueshifted by 17 km s$^{-1}$ with respect to the He 10830 rest wavelength, is detected in the spectrum of the Na-rich star, whereas the Na-poor star spectrum is consistent with a non-detection. The difference in the spectra is consistent and most closely explained by an He abundance difference between the two stars of $Delta Y ge$ 0.17.We provide direct evidence of a significant He line strength difference in giant stars of NGC 2808 belonging to different sub-populations, which had been previously detected by other photometric and spectroscopic means.
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