Diffuse X-rays from the Galactic center (GC) region were found to exhibit many K-shell lines from iron and nickel atoms in the 6--9 keV band. The strong emission lines seen in the spectrum are neutral iron K$alpha$ at 6.4~keV, He-like iron K$alpha$ at 6.7~keV, H-like iron Ly$alpha$ at 6.9~keV, and He-like iron K$beta$ at 7.8~keV. Among them, the 6.4~keV emission line is a probe of non-thermal phenomena. We have detected strong 6.4~keV emission in several giant molecular clouds, some of which were newly discovered by Suzaku. All the spectra exhibit large equivalent widths of 1-2~keV and absorption columns of $2-10times 10^{23}{rm H cm}^{-2}$. We found time variability of diffuse 6.4~keV emission in the Sgr B2 region comparing the maps and spectra obtained from 1994 to 2005 with ASCA, Chandra, XMM-Newton and Suzaku. We also report discovery of K$alpha$ lines of neutral argon, calcium, chrome, and manganese atoms in the Sgr~A region. We show that the equivalent width of the 6.4~keV emission line detected in X-ray faint region against the 6.4 keV-associated continuum (power-law component) is $sim 800 {rm eV}$. These features are naturally explained by the X-ray reflection nebula scenario rather than the low energy cosmic-ray electrons scenario. On the other hand, a 6.4~keV clump, G~0.162$-$0.217, discovered at the south end of the Radio Arc has a small equivalent width of 6.4~keV emission line of $sim200 {rm eV}$. The Radio Arc is a site of relativistic electrons. Thus, it is conceivable that the X-rays of G~0.162$-$0.217 are due to low energy cosmic-ray electrons
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