Spectroscopic long-slit observations of the dwarf Irr galaxy IC 10 were conducted at the 6-m Special Astrophysical Observatory telescope with the SCORPIO focal reducer. The ionized-gas emission spectra in the regions of intense current star formation were obtained for a large number of regions in IC 10. The relative abundances of oxygen, N+, and S+ in about twenty HII regions and in the synchrotron superbubble were estimated. We found that the galaxy-averaged oxygen abundance is 12 + log(O/H) = 8.17 +- 0.35 and the metallicity is Z = 0.18 +- 0.14 Z_sun. Our abundances estimated from the strong emission lines are found to be more reliable than those obtained by comparing diagnostic diagrams with photoionization models.