A new calculation of nuclear enhancement factor, used in estimation of Galactic diffuse gamma-ray flux from proton-proton interaction in order to take account of heavy nuclei included in cosmic-rays and interstellar matter, is presented by use of a Monte Carlo simulator, DPMJET-3. A new value of 1.8-2.0 in the energy range of 6-1000 GeV/nucleon, slightly increasing with kinetic energy of projectile cosmic rays, is about 20% larger than previous estimates.
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