A universal characterization of interactions in few- and many-body quantum systems is often possible without detailed description of the interaction potential, and has become a defacto assumption for cold atom research. Universality in this context is defined as the validity to fully characterize the system in terms of two-body scattering length. We discuss universality in the following three contexts: closed-channel dominated Feshbach resonance, Efimov physics near Feshbach resonances, and corrections to the mean field energy of Bose-Einstein condensates with large scattering lengths. Novel experimental tools and strategies are discussed to study universality in ultracold atomic gases: dynamic control of interactions, run-away evaporative cooling in optical traps, and preparation of few-body systems in optical lattices.