We consider systems of magnetized D9 branes on orbifolds supporting N=1 gauge theories. In such realizations, the matter multiplets arise from twisted strings connecting different stacks of branes. The introduction of Euclidean 5-branes wrapped on the six-dimensional compact space leads to instanton effects. We examine the interplay between the annuli diagrams with an E5 boundary and the holomorphicity properties of the effective low-energy supergravity action which describes the system, including its instanton corrections. Mostly based on arXiv:0709.0245.