Three-body and four-body interactions have been directly measured in a colloidal system comprised of three (or four) charged colloidal particles. Two of the particles have been confined by means of a scanned laser tweezers to a line-shaped optical trap where they diffused due to thermal fluctuations. By means of an additional focused optical trap a third particle has been approached and attractive three-body interactions have been observed. These observations are in qualitative agreement with additionally performed nonlinear Poissson-Boltzmann calculations. Two configurations of four particles have been studied experimentally as well and in both cases a repulsive four-body interaction term has been observed.
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