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The force field of optical tweezers is commonly assumed to be conservative, neglecting the complex action of the scattering force. Using a novel method that extracts local forces from trajectories of an optically trapped particle, we measure the three dimensional force field experienced by a Rayleigh particle with 10 nm spatial resolution and femtonewton precision in force. We find that the force field is nonconservative with the nonconservative component increasing radially away from the optical axis, in agreement with the Gaussian beam model of the optical trap. Together with thermal position fluctuations of the trapped particle, the presence of the nonconservative force can cause a complex flux of energy into the optical trap depending on the experimental conditions.
Viscosity is an important property of out-of-equilibrium systems such as active biological materials and driven non-Newtonian fluids, and for fields ranging from biomaterials to geology, energy technologies and medicine. However, noninvasive viscosit
We propose to use optical tweezers to probe the Casimir interaction between microspheres inside a liquid medium for geometric aspect ratios far beyond the validity of the widely employed proximity force approximation. This setup has the potential for
A method for forming permanent three dimensional structures from colloidal particles using holographic optical trapping is described. Holographic optical tweezers (HOT) are used to selectively position charge stabilized colloidal particles within a f
By exerting mechanical force it is possible to unfold/refold RNA molecules one at a time. In a small range of forces, an RNA molecule can hop between the folded and the unfolded state with force-dependent kinetic rates. Here, we introduce a mesoscopi
We have developed a new in situ method to calibrate optical tweezers experiments and simultaneously measure the size of the trapped particle or the viscosity of the surrounding fluid. The positional fluctuations of the trapped particle are recorded w