We study the two galaxies NGC4621 and NGC4374 in the Virgo cluster to derive their distances and stellar population properties. The targets have hosted three type Ia Supernova events allowing to investigate the correlations between the SNeIa and their host stellar systems. Using deep BVR data, obtained with FORS2 at the VLT, we analyse the Surface Brightness Fluctuations (SBF) properties of the targets. We adopt our measurements and existing calibrations to estimate the distance of NGC4621 and NGC4374. For stellar population analysis, we measured SBF amplitudes in different galaxy regions. We present a detailed comparison between data and models to constrain the characteristics of the dominant stellar components at i) various galactic radii, and ii) in the regions where SNeIa were recorded. Our V and R SBF measures provide distances in agreement with literature estimates. The median of our and literature SBF-based distances agrees with the one from non-SBF methods. Comparing data with models we find that stellar populations properties do not change significantly along galactic radius, with a dominant population having old age and solar chemical composition. The galaxies appear similar in all properties analysed, except for B-band SBF. Since the SBF magnitudes in this band are sensitive to the properties of a hot stellar component, we speculate that such behaviour is a consequence of different diffuse hot components in the galaxies. We find that the presence of a percentage of hot-HB stars in old and metal rich stellar populations could be at the origin of the observed differences. We find a good uniformity in the V and R SBF and integrated colours in the regions where the three SNeIa exploded. On the other hand, the B-band SBF signal shows intriguing differences.