The genus Potyvirus is one of the most important plant viral genera, as it contains about 200 virus species, some of which are of great importance. Our study showed the wide spread of viruses belonging to this genus in different regions of Syria and during successive seasons, and the infection rate was 54.25% on the plants of the cucurbit family, and in the samples of the Solanaceae family 34.17%, while the cruciferous family was at a rate of 57.57%, which indicates the presence of these viruses and their transmission from one season to another.