In the framework of this research, bacteria belonging to the genus Bacillus were isolated from some Syrian soils in the governorate of Damascus and its countryside, and the governorates of Daraa and Quneitra. Some morphological, agricultural and biochemical traits of the colonies were studied and the isolates producing cellulase enzyme were selected. The average diameters of the halos of the produced isolates ranged between 1 and 3.6 cm and the analysis efficiency ranged between 1.1 and 4, 20 out of 65 isolates producing the enzyme were classified using the (API 50 CH) system. DNA using a modified reference method.