The research was conducted in 2018 at Jeb Ramleh Research Station - Al-Ghab Research Center - General Authority of Scientific Agricultural Research The objective was to investigate the effect of three types of organic and biological fertilizers ( Bacillus , Humic acid and amino acids) , and four fertilization methods ( without , soaking , seeds, Plant irrigation, and leaf spraying ) and their interactions in some physiological traits of cotton line 124.