We present a systematic investigation of rotation curves (RCs) of fully hydrodynamically simulated galaxies, including cooling, star formation with associated feedback and galactic winds. Applying two commonly used fitting formulae to characterize the RCs, we investigate systematic effects on the shape of RCs both by observational constraints and internal properties of the galaxies. We mainly focus on effects that occur in measurements of intermediate and high redshift galaxies. We find that RC parameters are affected by the observational setup, like slit misalignment or the spatial resolution and also depend on the evolution of a galaxy. Therefore, a direct comparison of quantities derived from measured RCs with predictions of semi-analytic models is difficult. The virial velocity V_c, which is usually calculated and used by semi-analytic models can differ significantly from fit parameters like V_max or V_opt inferred from RCs. We find that V_c is usually lower than typical characteristic velocities derived from RCs. V_max alone is in general not a robust estimator for the virial mass.
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