Knowledge of the beam profiles is of critical importance for interpreting data from cosmic microwave background experiments. In this paper, we present the characterization of the in-flight optical response of the WMAP satellite. The main beam intensities have been mapped to < -30 dB of their peak values by observing Jupiter with the satellite in the same observing mode as for CMB observations. The beam patterns closely follow the pre-launch expectations. The full width at half maximum is a function of frequency and ranges from 0.82 degrees at 23 GHz to 0.21 degrees at 94 GHz; however, the beams are not Gaussian. We present: (a) the beam patterns for all ten differential radiometers and show that the patterns are substantially independent of polarization in all but the 23 GHz channel; (b) the effective symmetrized beam patterns that result from WMAPs compound spin observing pattern; (c) the effective window functions for all radiometers and the formalism for propagating the window function uncertainty; and (d) the conversion factor from point source flux to antenna temperature. A summary of the systematic uncertainties, which currently dominate our knowledge of the beams, is also presented. The constancy of Jupiters temperature within a frequency band is an essential check of the optical system. The tests enable us to report a calibration of Jupiter to 1-3% accuracy relative to the CMB dipole.