We present first room-temperature thermoelectric signature of the skyrmion lattice. This was observed in Fe3Sn2, a Kagome Dirac crystal with massive Dirac fermions that features high-temperature skyrmion phase. The room-temperature skyrmion lattice shows magnetic-field dependence of the wavevector whereas thermopower is dominated by the electronic diffusion mechanism, allowing for the skyrmionic bubble lattice detection. Our results pave the way for the future skyrmion-based devices based on the manipulation of the thermal gradient.