Subscribe to the gold package and get unlimited access to Shamra Academy
Register a new userSingle-shot hybrid photoacoustic-fluorescent microendoscopy through a multi-mode fiber with wavefront shaping
92
0
0.0
(
0
)
Ask ChatGPT about the research
No Arabic abstract
We present a minimally-invasive endoscope based on a multimode fiber that combines photoacoustic and fluorescence sensing. From the measurement of a transmission matrix during a prior calibration step, a focused spot is produced and raster-scanned over a sample at the distal tip of the fiber by use of a fast spatial light modulator. An ultra-sensitive fiber-optic ultrasound sensor for photoacoustic detection placed next to the fiber is combined with a photodetector to obtain both fluorescence and photoacoustic images with a distal imaging tip no larger than 250um. The high signal-to-noise ratio provided by wavefront shaping based focusing and the ultra-sensitive ultrasound sensor enables imaging with a single laser shot per pixel, demonstrating fast two-dimensional hybrid imaging of red blood cells and fluorescent beads.
rate research
Read More
Non-invasively focusing light into strongly scattering media, such as biological tissue, is highly desirable but challenging. Recently, wavefront shaping technologies guided by ultrasonic encoding or photoacoustic sensing have been developed to address this limitation. So far, these methods provide only acoustic diffraction-limited optical focusing. Here, we introduce nonlinear photoacoustic wavefront shaping (PAWS), which achieves optical diffraction-limited (i.e. single-speckle-grain) focusing in scattering media. We develop an efficient dual-pulse excitation approach to generate strong nonlinear photoacoustic (PA) signals based on the Grueneisen memory effect. These nonlinear PA signals are used as feedback to guide iterative wavefront optimization. By maximizing the amplitude of the nonlinear PA signal, light is effectively focused to a single optical speckle grain. Experimental results demonstrate a clear optical focus on the scale of 5-7 micrometers, which is ~10 times smaller than the acoustic focus in linear dimension, with an enhancement factor of ~6000 in peak fluence. This technology has the potential to provide highly confined strong optical focus deep in tissue for microsurgery of Parkinsons disease and epilepsy or single-neuron imaging and optogenetic activation.
We report experimental results, showing that the Kerr beam self-cleaning of many low-order modes in a graded-index multimode fiber can be controlled thanks to optimized wavefront shaping of the coherent excitation beam. Adaptive profiling of the transverse input phase was utilized for channeling the launched power towards a specific low-order fiber mode, by exploiting nonlinear coupling among all guided modes. Experiments were carried out with 7 ps pulses at 1064 nm injected in a five meters long multimode fiber operating in the normal dispersion regime. Optimized Kerr beam self-cleaning of five different LP modes is reported, with a power threshold that increases with the mode order.
Light scattering limits the penetration depth of non-invasive Raman spectroscopy in biological media. While safe levels of irradiation may be adequate to analyze superficial tissue, scattering of the pump beam reduces the Raman signal to undetectable levels deeper within the tissue. Here we demonstrate how wavefront shaping techniques can significantly increase the Raman signal at depth, while keeping the total irradiance constant, thus increasing the amount of Raman signal available for detection.
Photonic devices rarely provide both elaborate spatial control and sharp spectral control over an incoming wavefront. In optical metasurfaces, for example, the localized modes of individual meta-units govern the wavefront shape over a broad bandwidth, while nonlocal lattice modes extended over many meta-units support high quality-factor resonances. We experimentally demonstrate dielectric metasurfaces that offer both spatial and spectral control of light, realizing a metalens focusing light only over a narrowband resonance while leaving off-resonant frequencies unaffected. Our devices realize such functionality by supporting a quasi-bound state in the continuum encoded with a spatially varying geometric phase. We also show that our resonant metasurfaces can be cascaded to realize hyperspectral wavefront shaping, which may prove useful for augmented reality glasses, transparent displays and high-capacity optical communications.
We show that the output mode of a single-mode optical fiber can be directly focused to a sub-wavelength waist with a finite working distance by tapering the fiber to a diameter of the order of the wavelength and terminating it with a spherically/hemispherically shaped tip. Numerical simulations show that a beam waist with a width of as small as 0.62lambda can be formed. We fabricate micro-lensed fibers and construct a probe-scanning confocal reflection microscope. Measurements on gold nano-particles show a spatial profile with a width of 0.29lambda for lambda = 850 nm, which is in good agreement with the numerical simulations. Due to their monolithic structures, these micro-lensed fibers will be flexible substitutes for conventional compound lenses in various experimental conditions such as cryogenic temperature and ultra-high vacuum.
Log in to be able to interact and post comments
comments
Fetching comments
Sign in to be able to follow your search criteria
