We study the prospect of probing electroweak baryogenesis driven by an extra bottom Yukawa coupling $rho_{bb}$ in a general two Higgs doublet model via electric dipole moment (EDM) measurements and at the collider experiments. The parameter space receives meaningful constraints from 125 GeV Higgs $h$ boson signal strength measurements as well as several heavy Higgs boson searches at the Large Hadron Collider (LHC). In addition, we show that the asymmetry of the CP asymmetry of inclusive $Bto X_s gamma$ decay would provide complementary probe. A discovery is possible at the LHC via $bgto bA to b Z h$ process if $|rho_{bb}|sim 0.15$ and $250~mbox{GeV}lesssim m_A lesssim 350$ GeV, where $A$ is CP odd scalar. For $m_A> 2 m_t$ threshold, where $m_t$ is the top quark mass, one may also discover $bgto bA to b t bar t$ at the high luminosity LHC run if an extra top Yukawa coupling $|rho_{tt}|sim0.5$, though it may suffer from systematic uncertainties. For completeness we study $ggto t bar t A to t bar t b bar b $ but find it not promising.
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