Quantum computation, aiming at tackling hard problems beyond classical approaches, has been flourishing with each passing day. Unfortunately, a fully scalable and fault-tolerant universal quantum computer remains challenging based on the current technology. Boson sampling, first proposed by Aaronson and Arkhipov, is commonly believed as the most promising candidate to reach the intermediate quantum computational milestone, namely, quantum supremacy. Following this leading proposal, many experimental implementations as well as variants of boson sampling have been shown. However, most of these works are limited to small scale and cannot fulfill the permanent-of-Gaussians conjecture. Here, we experimentally demonstrate the largest scale boson sampling in the collision-free dominant regime using multi-port interferometer in a 3D photonic chip. We measure all 6,545 no-collision output combinations and validate the experimental results. Our work shows the potential of 3D photonic chip platform and represents a solid step toward large scale boson sampling.