We study the innermost stable circular orbit (ISCO) of a spinning test particle moving in the vicinity of an axially symmetric rotating braneworld black hole (BH). We start with the description of the event horizon, static limit surface and ergosphere region of such BH and bring out the effect of tidal charge parameter on ergosphere. It is found that the ISCO of rotating braneworld BH is very sensitive to braneworld BH parameter C (also known as tidal charge parameter) in addition to its rotation parameter. We further discovered that the orbital radius of the spinning test particles changes non monotonously with the braneworld BH tidal charge parameter. It is found that for rotating braneworld BH the allowed range of the particle spin grows as the tidal charge parameter C decreases, in contrast with the Kerr Newman BH. We also found the similar behavior of the particles spin for the braneworld Reissner Nordstrom (C < 0) BH in contrast with its counterpart having (C > 0).