Light scattering limits the penetration depth of non-invasive Raman spectroscopy in biological media. While safe levels of irradiation may be adequate to analyze superficial tissue, scattering of the pump beam reduces the Raman signal to undetectable levels deeper within the tissue. Here we demonstrate how wavefront shaping techniques can significantly increase the Raman signal at depth, while keeping the total irradiance constant, thus increasing the amount of Raman signal available for detection.
Download