Folding mechanisms are zero elastic energy motions essential to the deployment of origami, linkages, reconfigurable metamaterials and robotic structures. In this paper, we determine the fate of folding mechanisms when such structures are miniaturized so that thermal fluctuations cannot be neglected. First, we identify geometric and topological design strategies aimed at minimizing undesired thermal energy barriers that generically obstruct kinematic mechanisms at the microscale. Our findings are illustrated in the context of a quasi one-dimensional linkage structure that harbors a topologically protected mechanism. However, thermal fluctuations can also be exploited to deliberately lock a reconfigurable metamaterial into a fully expanded configuration, a process reminiscent of order by disorder transitions in magnetic systems. We demonstrate that this effect leads certain topological mechanical structures to exhibit an abrupt change in the pressure -- a bulk signature of the underlying topological invariant at finite temperature. We conclude with a discussion of anharmonic corrections and potential applications of our work to the the engineering of DNA origami devices and molecular robots.