We explore the breaking of Lorentz and CPT invariance in strong interactions at low energy in the framework of chiral perturbation theory. Starting from the set of Lorentz-violating operators of mass-dimension five with quark and gluon fields, we construct the effective chiral Lagrangian with hadronic and electromagnetic interactions induced by these operators. We develop the power-counting scheme and discuss loop diagrams and the one-pion-exchange nucleon-nucleon potential. The effective chiral Lagrangian is the basis for calculations of low-energy observables with hadronic degrees of freedom. As examples, we consider clock-comparison experiments with nuclei and spin-precession experiments with nucleons in storage rings. We derive strict limits on the dimension-five tensors that quantify Lorentz and CPT violation.