The equation of state of asymmetric nuclear matter is still controversial, as predictions at subsaturation as well as above normal density widely diverge. We discuss several experimental results measured in heavy-ion collisions with the INDRA array in the incident energy range 5-80 MeV/nucleon. In particular an estimate of the density dependence of the symmetry energy is derived from isospin diffusion results compared with a transport code: the potential part of the symmetry energy linearly increases with the density. We demonstrate that isospin equilibrium is reached in mid-central collisions for the two reactions Ni+Au at 52 MeV/nucleon and Xe+Sn at 32 MeV/nucleon. New possible variables and an improved modelization to investigate symmetry energy are discussed.
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