We present here the preliminary results of the study of the fluorescent iron line emission from X-ray pulsars with Be companions. We propose to use properties of this emission to investigate the spatial distribution and physical conditions of the matter around the compact object as well as in the binary system as a whole. Using data of the RXTE observatory the iron line behavior in the transient X-ray pulsar V 0332+53 spectrum was studied during the powerful type II outburst in 2004-2005. Particularly, we investigated a variability of the iron line equivalent width on different time scales (pulse period, orbital period, outburst phase) and searched for its correlation with the continuum flux, spectral parameters, etc.