EBV virus is a herpes virus which affects human and causes Mononucleosis Infection and may play a role in lymphoma. lymphoma has two types (H or NH) This study was performed between 2016-2017 at Tishreen University Hospital – Oncology department; we took blood samples and performed ELISA Test (Anti EBNA IgG) to all patients with lymphoma. 57 patients (31 male 54.4% and 26 female 45.6%) with lymphoma. Sex doesn't has statistical significance on lymphoma 25-50 year was the most age group affected by lymphoma We have 26 patients with Hodgkin lymphoma 45.6% and 31 patient with nonHodgkin lymphoma 54.4% In our study EBV disease found to have a role in causing lymphoma (P=0.004) but it doesn't cause a specific type of lymphoma (P=0.06). We noticed a relationship between the type of Hodgkin lymphoma and EBV disease (P=0.034) especially Nodular sclerosis and Mixed cellularity type but we didn't notice this relationship in nonhodgkin lymphoma.