Objective: Evaluate sensitivity and specificity of nitrite test to urine culture(gold standard) for diagnosing urinary tract infection in patients with sickle cell anemia with urinary symptoms and signs. Methods: The study sample included 79 children aged 2 to 14 years with SCA presenting with fever (temperature >38°C),abdominal pain, urgency, dysuria or increased urinary frequency who referral to the general pediatric clinic and the children's ambulance department in Tishreen University Hospital in Lattakia during( 2016-2017). Midstream urine specimen was collected from children. Samples were subjected to nitrite test, culture and sensitivity. Results : There was significant bacteriuria in 17 (21.5%) children with SCA. A positive test for nitrite was obtained in 12 of the 17(70.5%) children. The nitrite test has a specificity of 95.2% in detecting bacteriuria, a sensitivity of 70.6%, a positive predictive value of 80% and a negative predictive value of 92%. A positive nitrite test was significantly associated with bacteriuria, while a negative test was also significantly associated with an absence of bacteriuria.