The present investigation was carried out at the nursery of Agricultural faculty- Tishreen University, during 2016-2017, to evaluate the best organic culture medium for the seedling production of Zinnia (Zinnia elegans) and Stock (Matthiolaincana) with the lowest costs. Seven different mediums were experimented (Turf, waste mushroom compost, farm waste compost and their combinations). The results showed some differences in chemical and physical characters of experimented mediums. This differences had an impact on germination parameters (seed germination percentage, speed of germination, daily germination) and seedling quality (seedling length, collar diameter, fresh and dray weight, percentage of dray matter). The results indicated the mediums consisting of turf (T1), waste mushroom compost with turf (T6) and waste mushroom compost with farm waste compost (T7) gave the best germination rate and the highest quality of seedling. Data showed as well that T3 and T7 treatments realized the lowest costs and higher economic profit than other treatments.