The research was carried out at Jeb Ramlah Research Station – AlGhab Research Center - General Authority of Scientific Agricultural Research at 2017 growing season. The objective was to investigate the effect of three organic and bio fertilizers (Bacillus, Humic acid and Amino acids) and three fertilization methods (Soaking seeds, Vegetative spraying and Plant irrigation) in some morphological and production traits of cotton cultivar 124 (Gossypium herutum L.) The experiment was conducted according to RCBD with three replications. The first factor (fertilizer type) occupied the main plots, while the second factor (fertilization method) occupied the split plots. Statistical analysis was carried out using the Genestat.12 software and the least significant difference L.S.D. at 5% level was calculated. Studied traits included (at the beginning of Blooming stage): Plant Height, Plant wet weight, Plant dry weight, Leaf area and plant production of cotton. The results showed that the average of treatment of amino acids was superiority significant on the others in terms of plant height (70.70cm), Plant wet and dry weight (201.50, and 83.41g), respectively, Leaf area (4240.15cm2/plant) and plant production of cotton (75.13g). For fertilization method, Vegetative spraying average was superiority significant on the others in terms of all studied traits.