Experiments were conducted to study effect of radiation and salt levels on plant regeneration from callus for two cultivars of potato i.e. Riviera and Burren, under in vitro condition. Results showed that the efficiency of radiation in induced regeneration from callus in Riviera cultivar at salt level of (10 dS m-1) and Burren cultivar at salt levels (8, 12 dS m-1). For the purpose of making sure inheritance of salinity tolerant, mutant clones (plants induced from salt tolerant calli, which planted at salt levels of 8, 10, 12 dS m-1 and plants induced from non salt tolerant calli, which planted at salt level of 6 dS m-1) and their parental cultivars (Riviera and Burren) by exposing to salt stress conditions and comparing them with control (6 dS m-1). Results revealed that the lowest percentage of reduction in plant height, number of nods per plant and tuberization were observed in salt tolerant mutants. Plant height and number of nodes/plant can be considered as selective morphological markers for in vitro salt tolerance .