The research was conducted at Faculty of Agriculture, Damascus University/ Syria, with the help of Scientific Agricultural Research Center in Latakia during 2013 and 2014 seasons. Three rootstocks were used i.e. Sour orange, citrumelo and troyer citrange, to study some factors affecting the success of micrografting technique (concentrations and periods of sterilization, rootstock, shoot tip size, type of nutritive medium and the grafting method). The nutritive medium was prepared, and the shoot tips was isolated from Navel orange source, then the micrografting was done for the three rootstocks.