The wide geographical spread and the exponential growth of the numbers of goats around the world clearly demonstrate the ability of these ruminants to adapt to harsh climates and grazing land. The aim of this study was to assess the genetic diversity of 42 samples of Syrian goats from many domestication stations including Jabali, Shami and hybrid (hybridization between both Shami and Jabali). The study was done by DNA extraction of these samples, application of SSR technology using 7 microstellite markers. The alleles number of markers were 29 alleles, at a rate of 4.1 allele for each genetic locus. The number of alleles of each locus ranged from 3 alleles in genetically markers (BMS1714, INRAD07, SRCRSP09) to 8 alleles at the genetic marker SRCRSP01. The rate of allele-frequency across all studied genetic sites ranged from 0.071429 with the molecular size of 210 bp for the McM527 genetic site to 0.97619 with a molecular size of 55 bp for the SRCRSP09 genetic site.