This investigation was carried out at Tissue Culture Laboratory, Department of Horticulture, University of Mosul, Iraq during 2012-2014) on Catharanthus roseus Variety; Nirvana Pink Blush, which is one of the important medicinal plant, containing a group of alkaloids. Parts of leaves, nodes, and internodes were cultured on MS medium supplemented with NAA for callus initiation, data were taken after 28-day results refer that highest weight of callus; 0.612g, 0.639g ,0.835g were obtained from cultivated parts of leaves, nodes and internodes on MS medium supplemented with 1.0mg/l NAA respectively. Callus was initiated and cultured using parts of leaves, nodes and internodes on MS medium supplemented with BA and 1.0 mg/l of NAA, after 50 days.