The research was performed by isolating some species of soil- fungi from agricultural soils handling with different pesticides and fungicides. Where the following fungi were isolated: Aspergillus niger (The isolates A1 and A2), Fusarium oxysporum (The isolate F), Trichoderma harzianum (The isolate T). Also F. solani (The isolate F7) and T. viride (The isolate T.v) were used, whereas they were taken from high studies laboratory- Science Faculty- Tishreen University at a previous search. The research was conducted to assay the ability of the previous fungi to degradation a number of pesticides, the insecticide "Dolan", the insecticide "Plantocide", the nematicide "Vydate" and the fungicide "Bayfidan", through the effect of double dose of each pesticides in the biomass of the studied fungi on PDB medium. In addition to analysis the results residues of pesticides using Spectrophotometer, and evaluation the pH values.