The extracted oil from Gundelia Tournefortii seeds, which belongs to Asteraceae family and which is known as one of the medical herbal plants that is widely spread in the Syrian environment, was isolated using soxhlet continuous extraction. It's chemical composition was determined by GC/MS (Gas Chromatography – Mass Spectrometry). The analysis led to identification of 20 components. The extracted oil was characterized by high content of Linoleic Acid (52.9%), Palmitic Acid (20.1%) and Squalene (10.1%).