Extraction and Determination of Polyphenols in Dry Olive Leaves using Ultrasound Apparatus


Abstract in English

Total Polyphenol was studied in olive leaves collected from Lattakia area (AlQurdaha zone), where Phenolic compounds were extracted from dry Olive leaves by using two extraction methods: maceration and Ultrasonic Device. By the both two methods a study was carried out on the extraction solvent concentration effect where the extraction was done by using the mixture of Ethanol-Water with different percentages (60,70,80 %). As well, The extraction temperature effect was studied at the both methods at (20,30, 40°C); in addition to the study of the extraction time effect. At the method of Maceration Extraction, the total polyphenols amount was studied in dry olive leaves after extraction during different times (24,48,72 h). While at the Ultrasonic Extraction the times were (10,20,30 min). The study showed that the highest amount of phenolic compounds were existed in dry olive leaves which were extracted by Ultrasonic Waves with the concentration of the solvent ethanol-water 80%, temperature of 40°C, and with an extraction time of 20min.

References used

Ibrahim, E, H., Abdelgaleel, M,A., Salama, A. A., Metwalli, S, M. Chemical and Nutritional Evaluation of Olive Leaves and Selection the Optimum Conditions for Extraction their Phenolic Compounds, Agric. Res. Kafr El-Sheikh Univ, Egypt, 42 (1), 445-459, 2016
Pereira,A,P., Ferreira,I,C,F,R., Marcelino,F., Valentao,P., Andrade,P,B., Seabra,R., Estevinho,L., Bento,A., Pereira,J,A. Phenolic Compounds and Antimicrobial Activity of Olive (Olea Europaea L. Cv. Cobrançosa) Leaves. Molecules, Portugal, (12): 1153-1162, 2007
Herrero,M., Temirzoda,T,N., Segura-Carretero,A., Quirantes,R., Plaza,M., Ibañez,E. New Possibilities for the Valorization of Olive Oil By-Products. Journal of Chromatography A. 2011

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