This research was carried out in molecular biology laboratory faculty of agriculture /Damascus University during the agriculture season 2015-2016, to study the genetic diversity and determine the degree of genetic similarity among eleven 11 cultivated vetch (Aleppo, Idleb, Kamishly, Hassekeh, Tartous, Lattakia, Homs, Hama, Sweida, Damascus,Daraa) by using the technique ISSR.