Genomic DNA extraction from small volume specimens using different methods and assessment its concentration and purification
published by Aِl-Baath University
in 2016
in
and research's language is
العربية
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Abstract in English
DNA was extraction from scanning microscopic slides to
identification leishmaniases. Three methods used to DNA
extraction, first one using ready extraction solvent (Promega kit),
second depended on phenol-chloroform-iso propanol , whereas third
one depended on columns with silica gel membranes (Qia gen kit).
The results showed that (Promega kit) method didn’t gave high
concentration of DNA. (Qia gen kit) method was the best for DNA
concentration and purity
References used
Alger J, Martha C Acosta, Caterin Lozano, Carmelita Velasquez, Luz Angela Labrada.(1996) Stained Smears as a Source of DNA. Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 91(5): 589-591
Al-Nahhas Samar and Kaldas Rania . (2013): Characterization of Leishmania Species Isolated from Cutaneous Human Samples from Central Region of Syria by RFLP Analysis. ISRN Parasitology. Article ID 308726, 5 pages
Desjeux P 2004. Leishmaniasis: current situation and new perspectives. Comp Immunol Microbiol Infect Dis 27: 305-318