According to the algae ability in photosynthesis and carbohydrate production with high value in nutrition and industry, this research was carried out during 2015 to study the qualitative and quantitative content of carbohydrates in Chlorella vulgaris which was isolated from southern Syrian fresh water, and to determine the effect of the growth conditions; temperature (15, 25, 35 C), sodium nitrate concentration (0.1, 0.25, 0.4 g/l) and pH (6, 7, 8) on that content. The results showed that the total content was in the range of 20% to 40%;with galactose and rhamnose dominating among the rest of sugars. The temperature 15°C was the best to get the greatest total content with the highest value of starch, cellulose, hemicellulose and pectin. In addition, the low nitrate concentration 0.1 g/l was combined with the highest content of pectin and its compounds, while the glucose content was not affected by the nitrogen concentration. Related to pH effect, the acidic media was proved to be the best for the highest carbohydrate content. Moreover, cellulose, starch, and pectin increased in the alkali medium over the rest of carbohydrates.