204 samples were collected from various body samples during the year 2016. These 204 samples distributed as follows: 133 from urine , 37 swabs of wounds and burns, 10 swabs from the ear and pharynx, 12 from feces, 8 from CSF, 4 from blood. This study distinctly showed that gram negative bacilli were the most isolates 136 (67%) while 68 isolates (33%) were gram positive bacteria . E. coli dominated (39.68%) of the total gram negative bacilli. This study distinctly showed an increase in the ratio of E. coli resistance to one or more types of antibiotics , we found that E. coli resistance to all antibiotics except specific and expensive antibiotics, and we found that its complete resistance to the second ,third and fourth generation of cephalosporins , resistance to specific and antibiotics such as Imipenem.