This research was conducted to study the effectiveness of the variations of magic latin square design, to reduce the value of the experimental error, in experiences of microbiological (Lactobacillus acidophilus), and to improve the activity of random rectangles as one source of the variations of magic latin square design. Where they were conducting a random distribution of (6) treatments supposed to (36) experimental unit test has repeated the process of distribution (150) times in order to obtain magic latin squares realized the conditions which show per treatments once in the row and once in the column, and once in each rectangle within the one design.