The research aims to examine the relationship between organizational culture on the one hand, and between knowledge management practices in Tishreen University, on the other hand, where the researcher distributed a questionnaire to a sample of the colleges in the University of Tishreen reached (205) questionnaire was recovered (222) to identify them, it was good of them use (158) questionnaire. To test the relationship, the researcher used the Student T test for one sample, as well as the Pearson correlation coefficient.